Antimitotic activity of a New Compound Isolated from the Flower of Prosopis juliflora
Author Affiliations
- 1 Center for Biotechnology, Department of Biological Sciences, Birla Institute of Technology and Science, Pilani, INDIA
Res. J. Recent Sci., Volume 1, Issue (6), Pages 22-26, June,2 (2012)
Abstract
A new compound was isolated from the flower of Prosopis juliflora and identified by mass, IR and NMR techniques. Its antimitotic activity was evaluated with the help of allium test. Onion root tips were subjected with 4 and 8 mg/ml concentrations of the compound, up to 48 hours for studying its effect on root mitosis. The roots were examined in permanent root tip squash preparations stained by the aceto-carmine. The results obtained confirmed that the compound have various effects on chromosomes and induced different mitotic abnormalities and structural aberration of chromosomes. Various chromosomal aberrations such as clumping and stickiness, fragmentation, C-mitotic effect, anaphase bridge were observed, which clearly showed the clastogenic, antimitotic and cytotoxic effect.
References
- De Flora S., Izzotti A., D’Agostini F. and Balansky R.M., Multiple points of intervention in the prevention of cancer and other mutationrelated diseases, Mut. Res.,, 480 – 481 (2001)
- Fonrose X., Ausseil F., Soleilhac E., Masson V. and David P., Parthenolide inhibits tubulin carboxypeptidase activity, Cancer Res., 67, 3371 – 3378 (2007)
- Iwasaki S., Antimitotic agents; Chemistry and recognition of tubulin molecule, Med. Res. Rev., 13, 183 – 198 (1993)
- Mangale S.M., Chonde S.G. and Raut P.D., Use of Moringa oleifera (drumstick) seed as natural absorbent and an antimicrobial agent for ground water treatment, Res.J.Recent Sci., 1(3), 31-40 (2012)
- Mondal D. and Mondal T., A review on efficacy of Azadirachta indica A. juss based biopesticides: an Indian perspective, Res.J.Recent Sci., 1(3), 94-99 (2012)
- Edelman M.J., Novel cytotoxic agents for nonsmall cell lung cancer, J. Thorac. Oncol.,, 752-755 (2006)
- Latha P.G. and Panikkar K.R., Chemoprotective effect of Ixora coccinea L. flowers on cisplatin induced toxicity in mice, Cancer Lett., 130, 197 – 202 (1998)
- Andrade L.F., Campos J.M.S. and Davide L.C., Cytogenetic alterations induced by SPL (spent potliners) in meristematic cells of plant bioassays, Ecotoxicol. Environ. Saf.,71, 706–710 (2008)
- Leme D.M. and Marin-Morales M.A., Chromosome aberration and micronucleus frequencies in Allium cepacells exposed petroleum polluted water – a case study, Mutat. Res., 650, 80–86 (2008)
- Havey M.J., Rabinowitch H.D., Currah L., Genome organization in Allium. In Allium Crop Science, (Eds.). pp. 59-79. Recent Advances, CABI Publishing, United Kingdom, (2002)
- Kura’s M., Nowakowska J., S´ liwin´ ska E., Pilarski R., Ilasz R., Tykarska T., Zobel A. and Gulewicz K., Changes in chromosome structure, Mitotic activity and nuclear DNA content from cells of Allium test induced by bark water extract of Uncaria tomentosa (Willd.) DC., J. Ethnopharmacol., 107, 211–221 (2006)
- Fiskesjo G., Allium test for screening chemicals; evaluation of cytological parameters. In Plants for Environmental Studies, Wang W., Gorsuch J. W., Hughes J.S. (Eds.) 307-333 CRC, Lewis Publishers, New York, (1997)
- Witkus and Berger C.A., Veratrine, a new polyploidy inducing agent, J. Heredity, 35, 129-133 (1944)
- Steineggeer E. and Levana A., Constitution and c-mitotic activity of isocolchicine, Hereditas, 33, 385-396 (1947)
- Badr A., Ghareeb A. and El-Din H.M., Cytotoxicity of some pesticides in mitotic cells of V. faba roots, Egyptian J. App. Sci., , 457-468 (1992)
- Saxena P.N., Chauhan L.K.S. and Gupta S.K., 2005, Cytogenetic effects of commercial formulation of cypermethrin in root meristem cells of Allium sativum: Spectroscopic basis of chromosome damage, Toxicol., 216,244-252 (2005)
